Evaluation of a Monitor Guided Nephelornetric System ByG. Wider Zentrallabor des Wilhelminenspitals der Stadt Wien

The most cpmmon parameters in the specific protein field, namely the immunoglobulins G, A and M were investigated on the recently developed Immuno Video Nephelometer System (IVNS). This System consists of the nephelometer, a microprocessor controlled program and a monitor screen, where instructions, Standard curve and results are displayed. Scattered light of immuno-complexes is measured at equilibrium after incubation of prediluted antigen-antibody mixtures. Data were compared with those obtained by radial immunodiffusion (R1D), rate nephelometry (Beckman Immunochemistry System-ICS) and immunoturbidimetfy (ENI-Gemsaec). Intrabatch Variation on the Immuno Video Nephelometer System was found to be good (CV 2.6-3.7%) and day to day Variation was satisfactory (CV 3.3-8.6%). There was also good correlation between the values found on the Immuno Video Nephelometer System and those of the other methods (correlation coefficients of 0:94-0.98). Instrumentation advantages, Operation procedure and necessity of antigen excess check are discussed in detail.